Composite

Part:BBa_K759012

Designed by: Kenta Mine   Group: iGEM12_HokkaidoU_Japan   (2012-09-20)
Revision as of 13:50, 3 October 2012 by Kenta (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

eCFP + E. coli aggregation (Ag43) device induced by pBAD promoter.

In the presence of L-arabinose, pBAD promoter is induced and then wildtype Antigen 43 (Ag43) protein and eCFP (engineered cyan fluorescent protein)is produced. The wildtype Ag43 have 6 PstI sites.

eCFP locates in the upstream of Ag43 for estimating the relationship between expressed protein amount and aggregation efficiency. But eCFP expression wasn't confirmed in our experiment.

See also BBa_K759001about aggregation function of this part.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2694
    Illegal NgoMIV site found at 3918
    Illegal NgoMIV site found at 4428
    Illegal NgoMIV site found at 4449
    Illegal AgeI site found at 979
    Illegal AgeI site found at 3444
    Illegal AgeI site found at 4188
    Illegal AgeI site found at 4602
    Illegal AgeI site found at 4844
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


[edit]
Categories
Parameters
None