Measurement

Part:BBa_K880003

Designed by: Josh Atkinson, Mike Ferguson, and Ben Parker   Group: iGEM12_Michigan   (2012-09-29)
Revision as of 13:41, 3 October 2012 by Jatk (Talk | contribs) (→‎A Utility for Assaying fim-Based Recombinases)

An Asymmetrically Digestible Reporter Utility for Assaying fim-Based Recombinases

-IRL K137010 + Sequence with AgeI cut-site K165006 + IRR K137008 Mi8

This is an asymmetrically digestible reporter, same orientation of inverted repeats as K137058 (external sites located internally of flip region)(IRL - “flip region” - IRR) for assaying fim-Based recombinase activity.

Asymmetrical_Digest_Reporter_Orientations.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 116
  • 1000
    COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None