Coding
BSMT1

Part:BBa_J45004:Design

Designed by: Boyuan Zhu   Group: iGEM06_MIT   (2006-06-07)
Revision as of 21:11, 8 June 2006 by Boyuanzhu (Talk | contribs) (→‎Design Notes)


SAM:benzoic acid/salicylic acid carboxyl methyltransferase I; converts salicylic acid to methyl sali


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 663
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 204


Design Notes

Just CDS taken from GenBank

Designed Forward Primer:
GTT TCT TCG AAT TCG CGG CCG CTT CTA GAG [ATG GAA GTT GTT GAA GTT]
LENGTH: 48
GC CONTENT: 45.8 %
MELT TEMP: 67.7 ºC


Designed Reverse Primer: GTT TCT TCC TGC AGC GGC CGC TAC TAG TA[T TAT TAA TTT ATT TTG GTC AA]
LENGTH: 50
GC CONTENT: 38.0 %
MELT TEMP: 64.9 ºC

Source

Species: Petunia x hybrida from GenBank (AY233465); (also available: A. thaliana and N. suaveolens)

References

    • <biblio>Underwood05 pmid=15849311</biblio>
    • <biblio>Negre03 pmid=14630969</biblio>