Part:BBa_K801001:Design

pTUM101 yeast shuttle vector with pTEF1 promoter

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal prefix found at 36
Illegal suffix found at 506
12
INCOMPATIBLE WITH RFC[12]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 36
Illegal NheI site found at 3771
Illegal SpeI site found at 507
Illegal PstI site found at 521
Illegal NotI site found at 42
Illegal NotI site found at 514
21
INCOMPATIBLE WITH RFC[21]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 36
23
INCOMPATIBLE WITH RFC[23]
Illegal prefix found at 36
Illegal suffix found at 507
25
INCOMPATIBLE WITH RFC[25]
Illegal prefix found at 36
Plasmid lacks a suffix.
Illegal XbaI site found at 51
Illegal SpeI site found at 507
Illegal PstI site found at 521
1000
INCOMPATIBLE WITH RFC[1000]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI.rc site found at 261
Illegal BsaI.rc site found at 1928
Illegal BsaI.rc site found at 2874
Illegal SapI site found at 845
Illegal SapI site found at 3026

All forbidden restriction sites have been removed from the plasmid backbone, making it RFC25 compatible

Based on invitrogen's pYES2 plasmid. For more information see the part information of pTUM100 (BBa_K801000).

Keywords:

Abbreviations:

Related BioBrick:

Other versions:

Quality control measures:
Test digestion using SpeI+ PstI

Backbone:
Resistance: Amp

Protein coding:

Enzymatic activity:

Cytotoxicity:

Safety notes:

Intellectual property:

Corresponding part author/authors:

Source:
Commercial system: pYES2, Invitrogen

Organism:
Designed for the following Chassis:Saccharomyces cerevisiae, Escherichia coli

Literature references:

Database references: