Part:BBa_K801095:Design

Naringenin - chalcone synthase (CHS) coding region

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 717
  Illegal BsaI.rc site found at 1138
  Illegal SapI.rc site found at 840
  

Plasmid (template for PCR) provided by John A. Morgan, School of Chemical Engineering, Purdue University, West Lafayette, Indiana, USA

http://www.ncbi.nlm.nih.gov/nucleotide/11096318/

PMID: 15932991

Keywords: Coumaryol-CoA, Coumaroyl-Coenzyme A, Xanthohumol, Biosynthesis, 4-coumarate-coenzyme A ligase, aromatic prenyltransferase

Abbreviations: CHS = naringenin-chalcone synthase

Design Notes

Related BioBrick:

Other versions: BBa_K801094: CHS + yeast consensus sequence

Related BioBricks: BBa_K801090, BBa_K801091, BBa_K801092, BBa_K801093, BBa_K801094, BBa_K801096, BBa_K801097, BBa_K801098

Cloning details:
Designed in RFC10/RFC25

Quality control measures:
Part was totally sequenced

Backbone:

• Backbone name: pSB1C3
• Resistance: Cp
• Copynumber: high

Protein coding:

• Protein: naringenin-chalcone synthase
• Tag: c-terminally fused Strep

Enzymatic activity:
EC-number: 2.3.1.74

Cytotoxicity:

Safety notes:

Intellectual property:

Corresponding part author/authors:

Source

Source:
Plasmid: pKS2µHyg-PAL-4CL-CHS, provided by John A. Morgan, Purdue University, USA

Organism:
Genesequence derived from Hypericum androsaemum

References

Literature references:
[http://www.ncbi.nlm.nih.gov/pubmed/15932991 Pubmed : Jiang, H., Wood, K.V., Morgan, J.A., 2005. Metabolic Engineering of the Phenylpropanoid Pathway in Saccharomyces cerevisiae. Appl. Environ. Microbiol. 71, 2962–2969]

Database references:
[http://www.ncbi.nlm.nih.gov/nuccore/AF315345.1 GenBank: Hypericum androsaemum chalcone synthase mRNA, complete cds]

[http://www.uniprot.org/uniprot/Q9FUB7 Uniprot: Chalcone synthase]