Coding

Part:BBa_K936032

Designed by: Sergio Castro Waqued   Group: iGEM12_UC_Davis   (2012-10-03)
Revision as of 08:38, 3 October 2012 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


LC Cutinase mutant S101A

Mutant of the Polyetheylene Terephtalate degrading protein LC Cutinase (Bba_K936000). The serine at the 101st amino acid position is changed to an alanine. This part was created using site directed mutagenesis to change a "TCG" codon to a "GCG" codon.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 600
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None