Translational_Unit
B0034-rhaS

Part:BBa_K902070:Design

Designed by: Chris Wintersinger   Group: iGEM12_Calgary   (2012-10-02)
Revision as of 08:13, 3 October 2012 by Cmwinter (Talk | contribs) (→‎Source)

B0034-rhaS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 458
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 190
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The rhaS gene with a strong RBS.

Please view our [http://2012.igem.org/Team:Calgary/Project/HumanPractices/Killswitch/Regulation#rhamnose Wiki] for more design details.

Source

RhaS was amplified from Top Ten E. coli with Kapa Hi-Fi polymerase. This part was then assembled with a strong RBS.

References