Coding
RhaS

Part:BBa_K902068:Design

Designed by: Chris Wintersinger   Group: iGEM12_Calgary   (2012-10-02)
Revision as of 07:42, 3 October 2012 by Cmwinter (Talk | contribs) (→‎Source)

RhaS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 440
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 172
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This serves as regulatory element which activates expression of the pBAD operon on the pRha rhamnose promoter. In order for it to bind to pRha, the crp-cAMP complex must be bound to the promoter. Additionally, rhamnose will improve function of RhaS (Egan & Schlief, 1993).

In its native E. coli, the rhaS (on the rhaSR operon off the pRha promoter) is upregulated by the RhaR-rhamnose complex (Egan & Schlief, 1993).

Please view our [http://2012.igem.org/Team:Calgary/Project/HumanPractices/Killswitch/Regulation#rhamnose Wiki] for more design details.

Source

PCR amplified from Top Ten E. coli with Kapa polymerase.

References

Egan, S. M., Schleif, R. F., & others. (1993). A regulatory cascade in the induction of rhaBAD. Journal of Molecular Biology, 234(1), 87-98.