Regulatory
polAP

Part:BBa_K847210

Designed by: Bryce Bajar, Jason Hu, Chris Jackson, Debha Amatya, Ben Geilich   Group: iGEM12_Stanford-Brown   (2012-07-24)
Revision as of 07:39, 3 October 2012 by Bbajar (Talk | contribs)

polA (DNA Polymerase I) DnaA-activated promoter

The polA promoter (polAP) is a DNA-replication dependent promoter.

Usage and Biology

DnaA is the central initiator of DNA replication in E. coli and other prokaryotic organisms, but importantly, it also functions as a transcription factor that can suppress or activate transcription of genes by binding to the DnaA box, a 9bp consensus sequence (Messer 1997). Since DnaA expression is dependent on the growth conditions of the cell (Chiaramello 1990), genes regulated by DnaA are transitively growth dependent. One such DnaA-dependent gene is polA, which codes for DNA Polymerase I, active in DNA replication (Quiñones 1997).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
//promoter
Parameters
biology
control