Regulatory
pRha

Part:BBa_K902065:Design

Designed by: Himika Dastidar, Chris Wintersinger   Group: iGEM12_Calgary   (2012-10-02)
Revision as of 07:07, 3 October 2012 by Cmwinter (Talk | contribs) (→‎Design Notes)

Rhamnose inducible, glucose repressible promoter (pRha)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This promoter was commercially synthesized from the sequence characterized by Jeske and Altenbuchner (2010).

The pRha promoter is two promoters in one. In its native E. coli, the 5' end of the promoter serves to express the rhaR and rhaS control genes. The 3' end of the promoter is expresses the rhaBAD rhamnose metabolism genes.

The rhamnose response: RhaR transcription factor is activated by rhamnose to upregulate rhaR and rhaS. RhaS in turn up-regulates the rhaBAD operon. These functions are dependent on the binding of the catabolite receptor protein (CRP) cAMP complex to the promoter. Additionally, function of RhaS is improved in the presence of rhamnose (Egan & Schleif, 1993).

The glucose response: The promoter is subject to repression via global catabolite repression.

Please see the details on our [http://2012.igem.org/Team:Calgary/Project/HumanPractices/Killswitch/Regulation Wiki].

Source

N/A

References