Regulatory
pRha

Part:BBa_K902065:Design

Designed by: Himika Dastidar, Chris Wintersinger   Group: iGEM12_Calgary   (2012-10-02)
Revision as of 06:46, 3 October 2012 by Cmwinter (Talk | contribs) (→‎Design Notes)

Rhamnose inducible, glucose repressible promoter (pRha)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This promoter was commercially synthesized from the sequence characterized by Jeske and Altenbuchner (2010).

The pRha promoter is two promoters in one. In its native E. coli, the 5' end of the promoter serves to express the rhaR and rhaS control genes. The 3' end of the promoter is expresses the rhaBAD rhamnose metabolism genes.

Source

N/A

References