Coding

Part:BBa_K902034

Designed by: Jeff Addison   Group: iGEM12_Calgary   (2012-10-01)
Revision as of 05:14, 3 October 2012 by Jeffaddison89 (Talk | contribs)

CarAd Coding Sequence

The carAd gene in Pseudomonas putida codes for the ferredoxin reductase component of carbazole-1,9-dioxygenase. Together with carAa and carAc it forms the carbazole-1,9-dioxygenase (CARDO) enzyme which converts carbazole into 2'-aminobiphenyl-2,3-diol by cleaving one C-N bond in the original carbazole molecule.
Action of the CarA enzyme complex.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 63
    Illegal SapI.rc site found at 51


[edit]
Categories
Parameters
None