Coding
fhlA-E363
Part:BBa_K778002
Designed by: Yuka Yamazaki Group: iGEM12_UT-Tokyo (2012-09-22)
fhlA-E363G (engineered trascriptional activator fhlA with a mutation)
FhlA is the transcriptional activator of hycAp Part:BBa K778000 .E.coli overexpressing FhlA produce H2 (since hycAp is the promoter of FHL(formate hydrogen lyase)).
fhlA-E363G has one point mutation (A to G, glutamic acid to glycine) from wild-type fhlA. This mutation increased H2 production under the conditions in the previous research (Viviana et.al.2009). Actually, in our research (team UT-Tokyo 2012[http://2012.igem.org/Team:UT-Tokyo/Project/H2_E.coli]), this mutation increased H2 production.
Detailed Information :[http://2012.igem.org/Team:UT-Tokyo/Project/H2_E.coli] but the results in this wiki is older than this figure above.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2096
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1375
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |