Regulatory

Part:BBa_K799007

Designed by: Jiarui Wang   Group: iGEM12_Johns_Hopkins-Wetware   (2012-09-23)
Revision as of 04:13, 3 October 2012 by Lmitch24 (Talk | contribs)

Ethanol/Stress Inducible YMR170C Promoter

YMR170C promoter shows an increase in protein expression when exposed to up to 14% ethanol.

YMR170C promoter is expected to drive gene expression in Saccharomyces cerevisiae in the presence of ethanol. We tested this by expressing GFP under the control of YMR170C promoter in the presence of multiple different concentrations of ethanol. We found an induction of GFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Characterization

ProA4 fluoro.png

This is fluorescence data for the expression of GFP under the control of YMR170C, our ethanol induced promoter. Fluorescence can be seen soon after ethanol induction. Furthermore, the fluorescence plateaus but remains significant till our last time point of 230 minutes.

Plasmid Map

YMR170C plasmid.png

Sequencing

ProA4 sequencing.png

This is the sequencing result for YMR170C promoter. As can be seen, it is a perfect match to the genomic DNA promoter.

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