Coding

Part:BBa_K799027

Designed by: Daniel Wolozny   Group: iGEM12_Johns_Hopkins-Wetware   (2012-09-25)
Revision as of 03:35, 3 October 2012 by Lmitch24 (Talk | contribs)

CYP2E1

CYP2E1 gene encodes the protein, Cytochrome P450 2E1 protein.It possesses a few functions such as its participation in the oxidative metabolism of a range of substrates like small polar molecules. Furthermore, it is able to convert ethanol to acetaldehyde and acetate in humans.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1482
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1302
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 1496


Plasmid Map

CYP2E1 plasmid.png

Sequencing

CYP2E1 sequencing.png

This is the sequencing result for the CYP2E1 human gene, codon optimized for S. cerevisiae expression. As can be seen, it is a perfect match to the expected sequence.

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Categories
Parameters
None