Coding
hpaC

Part:BBa_K902057

Designed by: Lisa Oberding, Ali Honarmand   Group: iGEM12_Calgary   (2012-10-01)
Revision as of 03:07, 3 October 2012 by Lisa.O (Talk | contribs)

HpaC- 4-hydroxyphenylacetate 3-monooxygenase reductase subunit

HpaC is an oxidoreductase capable of recycling FMN to FMNH2 while converting NADH to NAD. This part is used in conjuctions with the 4S desulfurization pathway to replace DszD. Doing so improves the rate of desulfurization 7-10x.


File:File.https://static.igem.org/mediawiki/2012/thumb/1/1c/Ucalgary team sulfur 4s enzyme pathway diagram.png/700px-Ucalgary team sulfur 4s enzyme pathway diagram.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 283


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Categories
Parameters
None