Terminator

Part:BBa_K799029

Designed by: Daniel Wolozny   Group: iGEM12_Johns_Hopkins-Wetware   (2012-09-25)
Revision as of 02:16, 3 October 2012 by Danielw9 (Talk | contribs)

MFA2 Terminator

Terminator of the MFA2 gene from Saccharomyces Cerevisiae. It confers relative instability to the mRNA produced, increasing the amount of mRNA made and protein produced. [[1]] "TPS1 Terminator Increases mRNA and Protein Yield in a Saccharomyces Cerevisiae Expression System"

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 217

Plasmid Map

MFA2t plasmid.png

Sequencing

MFA2t sequence.png

This is the sequencing result for the MFA2 gene terminator. As can be seen, it is a perfect match to the genomic DNA terminator.

[edit]
Categories
Parameters
None