Translational_Unit

Part:BBa_K741006

Designed by: Wuyang Chen   Group: iGEM12_USTC-China   (2012-07-24)
Revision as of 01:06, 3 October 2012 by MelodyChen (Talk | contribs)

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RBS-crogfp-T

It is a fusion protein of Cro and GFP. The linker between the two protein is GGSG (BBa_K648005). We use this part to test the ability of anticro to repress the expression of cro, for crogfp can both be repressed by asRNA of cro and emit green fluorescence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 81
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 875


[edit]
Categories
Parameters
None