Translational_Unit

Part:BBa_K741006:Design

Designed by: Wuyang Chen   Group: iGEM12_USTC-China   (2012-07-24)
Revision as of 01:04, 3 October 2012 by MelodyChen (Talk | contribs) (→‎Design Notes)

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RBS-crogfp-T


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 81
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 875


Design Notes

We construct crogfp by overlap PCR. The primers we design can combine cro and gfp with a linker [GGSG (BBa_K648005)] and add an RBS and a terminator to it.

Source

BBa_B0034 BBa_K741005 BBa_B0015

References