Coding

Part:BBa_K741005:Design

Designed by: Wuyang Chen   Group: iGEM12_USTC-China   (2012-07-24)
Revision as of 00:58, 3 October 2012 by MelodyChen (Talk | contribs) (→‎Design Notes)

Fusion Protein: crogfp


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 63
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 857


Design Notes

We construct this part by overlap PCR. The primer we design can combine cro and gfp with a linker [GGSG (BBa_K648005)] and add an RBS and a terminator to it.

Source

BBa_K648005 BBa_I13504

References