Generator
ccdB

Part:BBa_P1010:Design

Designed by: Leon Chan   Group: Antiquity   (2004-07-28)
Revision as of 19:34, 8 June 2006 by Randy (Talk | contribs)

ccdB cell death gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 664
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 114


ccdB

CcdB, a natural analogue of the quinolone antibiotics (e.g., ciprofloxacin, enoxacin, etc.), binds to the DNA gyrase subunit A, the product of the gyrA gene, turning it into a cellular poison (Bahassi, et al. 1999 J. Biol. Chem. 274: 10936-10944). E.coli strains that carry the gyrA462 mutation (e.g., DB3.1) are resistant to CcdB poisoning.