Plasmid_Backbone

Part:BBa_K799024

Designed by: Daniel Wolozny   Group: iGEM12_Johns_Hopkins-Wetware   (2012-09-25)
Revision as of 21:02, 2 October 2012 by Danielw9 (Talk | contribs)

pSB1C3 yGG promoter acceptor vector

The original psb1c3 backbone was modified in order to allow for Golden Gate enabled parts to easily become biobricks. Within the iGEM prefix and suffix, extra overhangs were added containing the appropriate Bsa1 sites for Golden Gate Assembly.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XhoI site found at 1012
    Illegal XhoI site found at 1904
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.



Plasmid Map

Promoters Acceptance Vector Plasmid Map.png

Sequencing

[[]]

[edit]
Categories
Parameters
None