Part:BBa_K901003

InvA Expression Construct

Coding sequence for Invertase A (BBa_K901000) under the control of a LacI promoter with a sRBS (BBa_J04500).

This expression construct is part of a 3 enzyme system designed to enhance the production and secretion of monosaccharide sugars in Synechococcus elongatus. This system is comprised of Galu (BBa_K901007), which codes for UDP-glucose phosphorylase. This is an enzyme from E. coli that produces UDP-glucose. The presence of UDP-glucose enhances the native production of the disaccharide sucrose in S. elongatus in response to salt shock.

Sucrose is then cleaved into glucose and fructose by Invertase A () and secreted from the cell via the membrane bound transport protein Glf (BBa_K901008). These monosaccharides are sufficient nutriment for E. coli and will be used to feed a culture of engineered cells designed to produce high levels of acetic acid.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 287
  Illegal NgoMIV site found at 1199
  
• 1000
  COMPATIBLE WITH RFC[1000]