Part:BBa_K833010
Mutated prfA-UTR thermosensor from Listeria monocytogenes
This part was derived from part BBa_K619889 via random mutagenesis and screening for thermosensors that responded to a 35 to 37'C shift. Part BBa_K619889 is a thermosensor for Listeria monocytogenes which normally responds to a 30' to 37'c shift. The thermosensor has a shine delgarno and initiating ATG that are barried within a stem-loop structure at 30'C, but are accessible at higher temperatures as the mRNA structure is disrupted. We monitored thermosensor activity via the reporter LacZ. This thermosensor is part of a library of thermosensors from this screen (see parts (Bba_K833000 to Bba_K833010 and also BBa_K619890,BBa_K61989 -619894 from our 2011 submission).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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