Regulatory

Part:BBa_K740000

Designed by: Lei Wei   Group: iGEM12_Tsinghua-A   (2012-09-23)
Revision as of 14:29, 2 October 2012 by CubicStone (Talk | contribs) (→‎Functional Parameters)

Constitutive promoter flanked by two loxP sites

This is a constitutive promoter (J23115) flanked by two opposite loxP sites so that it can flipped by Cre recombinase.

Usage and Biology

Cre-loxP is a site-specific system that can make a DNA sequence flipped. This part can make a constitutive promoter (J23115) flipped and it can work as a switch driven by Cre. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 41
    Illegal NheI site found at 64
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Functional Parameters

We constructed K740001 to test this part. The expression of RFP depends on the direction of the promoter. We transformed BBa_K740000 and BBa_I718008 into EPI300, and used flowcytometer to get the number of cells that expressed RFP.

We can got from this figure that the efficiency of this part depends on the expression of Cre. We can easily got a 80% efficiency with a short inducing time, while better may be able to gotten by some control methods, just like feedforward. K740000-F1.png

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Categories
Parameters
None