Regulatory

Part:BBa_K740000

Designed by: Lei Wei   Group: iGEM12_Tsinghua-A   (2012-09-23)
Revision as of 14:08, 2 October 2012 by CubicStone (Talk | contribs)

Constitutive promoter flanked by two loxP sites

This is a constitutive promoter (J23115) flanked by two opposite loxP sites so that it can flipped by Cre recombinase.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 41
    Illegal NheI site found at 64
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Cre-loxP is a site-specific system that can make a DNA sequence flipped. This part can make a constitutive promoter (J23115) flipped and it can work as a switch driven by Cre.


[edit]
Categories
Parameters
None