RNA

Part:BBa_K779117:Design

Designed by: Divya Israni   Group: iGEM12_MIT   (2012-09-30)
Revision as of 06:09, 2 October 2012 by Eerik (Talk | contribs) (→‎Design Notes)

RNA Reporter top strand with quencher (RQ) MammoBlock


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Modifications: 5' Iowa Black RQ quencher, all bases modified with 2'O Methyl (ordered as a DNA oligo with Us instead of Ts).

IDT DNA Oligo Order: /5IAbRQ/ mCmAmUmAmAmCmAmCmAmAmUmCmAmCmA

Source

Qian L., Winfree E. Scaling up digital circuit computation with DNA displacement cascades., Science. 2011 Jun 3; 332 (6034):1196-201

References