RNA

Part:BBa_K779117

Designed by: Divya Israni   Group: iGEM12_MIT   (2012-09-30)
Revision as of 06:09, 2 October 2012 by Eerik (Talk | contribs)

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RNA Reporter top strand with quencher (RQ) MammoBlock

Modifications: 5' Iowa Black RQ quencher, all bases modified with 2'O Methyl (ordered as a DNA oligo with Us instead of Ts).

IDT DNA Oligo Order: /5IAbRQ/ mCmAmUmAmAmCmAmCmAmAmUmCmAmCmA

RNA version of Part:BBa_K779121. Also see Part:BBa_K779120 for a modified version that includes a marker fluorophore.

Usage and Biology

Used along with Part:BBa_K779116 as part of an RNA reporter for strand displacement. See Part:BBa_K779120 and Part:BBa_K779116 for related experimental data.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aRNA Reporter top strand with quencher (RQ) MammoBlock