Coding

Part:BBa_K902034:Design

Designed by: Jeff Addison   Group: iGEM12_Calgary   (2012-10-01)
Revision as of 05:56, 2 October 2012 by Jeffaddison89 (Talk | contribs)

CarAd Coding Sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 63
    Illegal SapI.rc site found at 51


Design Notes

Site directed mutagenesis was required to remove a NotI site. Nucleotide 657 was changed from a G in the native DNA to an A in our construct. This was a conservative mutation which had no effect on the amino acid output.


Source

Pseudomonas putida plasmid pCAR1.2

References