Coding

Part:BBa_K799028

Designed by: Daniel Wolozny   Group: iGEM12_Johns_Hopkins-Wetware   (2012-09-25)
Revision as of 05:34, 2 October 2012 by Danielw9 (Talk | contribs) (→‎Plasmid Map)

typeIIS-less GFP

GFP modified to remove all BsaI sites.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Plasmid Map

GFP bsa- plasmid.png

Sequencing

GFP no bsa sequencing.png

This is the sequencing result for human codon optimized GFP. As can be seen, it is a perfect match to our expected sequence.

[edit]
Categories
Parameters
None