Coding

Part:BBa_K799027

Designed by: Daniel Wolozny   Group: iGEM12_Johns_Hopkins-Wetware   (2012-09-25)
Revision as of 05:30, 2 October 2012 by Danielw9 (Talk | contribs) (→‎Plasmid Map)

CYP2E1

...

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1482
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1302
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 1496


Plasmid Map

CYP2E1 plasmid.png

Sequencing

CYP2E1 sequencing.png

This is the sequencing result for the CYP2E1 human codon optimized gene. As can be seen, it is a perfect match to the genomic DNA promoter.

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