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Part:BBa_K779502

Designed by: Kristjan Eerik Kaseniit   Group: iGEM12_MIT   (2012-09-29)
Revision as of 05:25, 2 October 2012 by Eerik (Talk | contribs)

Long reporter bottom strand MammoBlock

Long reporter bottom strand to bind to Part:BBa_K779501 and Part:BBa_K779500.

Modifications: 3' ROX florophore, all bases modified with 2'O Methyl (ordered as a DNA oligo with Us instead of Ts).

IDT DNA Oligo Order: mUmGmGmAmUmAmGmUmUmGmGmUmGmGmGmAmGmAmGmGmAmGmUmGmGmGmUmG/3ROX_N/

This is an improved version of Part:BBa_K779116, with this design addressing the low melting temperature, orthogonality and small length of the previous iteration.

Usage and Biology

Provides a quenchable fluorophore. When annealed to a top strand (see parts above), the ROX fluorophore is quenched by an Iowa Black RQ quencher. When an input strand (Part:BBa_K779503) is added, strand displacement occurs and the quencher top strand is released.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aLong reporter bottom strand MammoBlock