![](https://parts.igem.org/images/partbypart/icon_other.png)
Other
Part:BBa_K779501
Designed by: Kristjan Eerik Kaseniit Group: iGEM12_MIT (2012-09-29)
Long reporter top strand MammoBlock
Long reporter top strand to be annealed with Part:BBa K779502.
Modifications: 5' Iowa Black RQ quencher, 3' Alexa 488 florophore, all bases modified with 2'O Methyl (ordered as a DNA oligo with Us instead of Ts).
IDT DNA oligo order: /5IAbRQ/mCmAmCmCmCmAmCmUmCmCmUmCmUmCmCmCmAmCmCmA/3AlexF488N/
Usage and Biology
When annealed to Part:BBa K779502 acts as a reporter for strand displacement reactions.
Sequence and Features
Sequence is designed to be orthogonal to the HEK293 transcriptome (see Design). Design is improved on the part Part:BBa_K779121, with goals being a higher melting temperature, more orthogonal and longer domain.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
n/a | Long reporter top strand MammoBlock |