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Part:BBa_K779501

Designed by: Kristjan Eerik Kaseniit   Group: iGEM12_MIT   (2012-09-29)
Revision as of 05:11, 2 October 2012 by Eerik (Talk | contribs)

Long reporter top strand MammoBlock

Long reporter top strand to be annealed with Part:BBa K779502.

Modifications: 5' Iowa Black RQ quencher, 3' Alexa 488 florophore, all bases modified with 2'O Methyl (ordered as a DNA oligo with Us instead of Ts).

IDT DNA oligo order: /5IAbRQ/mCmAmCmCmCmAmCmUmCmCmUmCmUmCmCmCmAmCmCmA/3AlexF488N/

Usage and Biology

When annealed to Part:BBa K779502 acts as a reporter for strand displacement reactions.


Sequence and Features

Sequence is designed to be orthogonal to the HEK293 transcriptome (see Design). Design is improved on the part Part:BBa_K779121, with goals being a higher melting temperature, more orthogonal and longer domain.



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aLong reporter top strand MammoBlock