Regulatory

Part:BBa_K799001

Designed by: Jiarui Wang   Group: iGEM12_Johns_Hopkins-Wetware   (2012-09-23)
Revision as of 04:51, 2 October 2012 by Danielw9 (Talk | contribs) (→‎Plasmid Map)

Ethanol/Stress Inducible YJL200C Promoter

YJL200C promoter shows an increase in protein expression when exposed up to 14% ethanol.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 172
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 172
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 172
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 172
  • 1000
    COMPATIBLE WITH RFC[1000]

Characterization

ProA1 fluoro.png

This is fluorescence data for the expression of GFP under the control of YJL200C, our ethanol induced promoter. Fluorescence can be seen soon after ethanol induction. Furthermore, the fluorescence plateaus but remains significant till our last time point of 230 minutes.

Plasmid Map

YJL200C plasmid.png

Sequencing

YJL200C sequencing.png

This is the sequencing result for YJL200C promoter. As can be seen, it is a perfect match to the genomic DNA promoter.

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Parameters
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