Coding

Part:BBa_K749004

Designed by: Takuya Akutsu   Group: iGEM12_TMU-Tokyo   (2012-09-26)
Revision as of 04:46, 2 October 2012 by T.acts (Talk | contribs)

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frmR, formaldehyde regulator protain

Biosafety_Level1.png Safety.png

The sequence of frmR contains amber suppressor codon (UAG) at its 3' end. Amber suppressor codon recognizes its specialized tRNA.
But the tRNA sometimes alters and gets capacity to carry amino acid. If amino acid comes, transcription is continued to more 7 amino acids and cording protein is degenerated. Wherein, frmR loses inhibitory.

Repressing ability of frmR was not shown molecularly but quantitatively. The downstream of frmR is inhibited by frmR and not by frmR added 7 amino acids.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 38
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None