RNA

Part:BBa_K779119

Designed by: Divya Israni   Group: iGEM12_MIT   (2012-09-30)
Revision as of 04:30, 2 October 2012 by Eerik (Talk | contribs)

RNA Control input (scrambled) MammoBlock

Used as a control input to the RNA reporter formed by parts Part:BBa_K779116 and (Part:BBa_K779120 or Part:BBa_K779117). Refer to these part pages for data on Part:BBa K779119.


Modifications: all bases modified with 2'O Methyl (ordered as a DNA oligo with Us instead of Ts).

IDT DNA Oligo Order: mCmAmUmCmCmAmUmUmCmCmAmCmUmCmA mUmCmU mCmA

Usage and Biology

Used in concert with the parts mentioned above, it acts as a negative control for a strand displacement reaction (see Part:BBa_K77912 and other related parts). While it has a matching toehold, the recognition domain is non-matching, so strand-displacement will not occur.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aRNA Control input (scrambled) MammoBlock