Part:BBa_K921001

T7 RNAP + IPTG->PoPs (Mutant II)
This is a mutant T7 promoter that includes a lacO sequence. This is repressible by the LacI protein and is induced in the presence of IPTG. The promoter sits in the 5' region of the gene of interest and initiates transcription when the cell expresses T7 RNA polymerase. Be sure you are using a cell strain that is compatible with T7 expression vectors.
The following parameters were calculated and compared against the "wild-type" [1] The following parameters were calculated:

Promoter	BBa K921000	BBa K921001	BBa K921002
Transcription Strength	97%	72%	127%
Translational Efficiency	6%	6%	94%

Fluorogen activating proteins were used as protein reporters in our characterization experiment as described here. Here, a saturating amount of malachite green was added to each well of a 96 well plate and fluorescence values were recorded over time. Our model was used to describe the behavior of our time lapse data using differential equations and fitting algorithms.


The Spinach aptamer was used as an RNA reporter in our characterization experiment as described here. Here, a saturating amount of DFHBI was added to each well of a 96 well plate and fluorescence values were recorded over time. Our model was used to describe the behavior of our time lapse data using differential equations and fitting algorithms.

Note: Not included here are the properties of our characterization method. To learn more about how we characterized this set of promoters, click here.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Functional Parameters

direction	Forward
efficiency	90%
negative_regulators	LacI (Hypothesized LacIQ compatible)