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Coding

Part:BBa_J45011

Designed by: Stephen Payne   Group: iGEM06_MIT   (2006-06-07)
Revision as of 14:26, 8 June 2006 by Stephen (Talk | contribs)

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PAL (Phenylalanine Ammonia-lyase) catalyzes the conversion of phenylalanine to cinnamic acid

Phenylalanine is a common chemical placed into media as food for E. coli. Phenylalanine can be converted to cinnamic acid in E. coli with the addition of this gene encoding Phenylalanine Ammonia-lyase (as done by Faulkner, et al). The sequence displayed is the initial gene from Rhodosporidium toruloides, complete with introns and exons. For more information, see http://openwetware.org/wiki/IGEM:MIT/2006/Notebook/2006-6-6.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2508
    Illegal BamHI site found at 476
    Illegal XhoI site found at 551
    Illegal XhoI site found at 588
    Illegal XhoI site found at 651
    Illegal XhoI site found at 669
    Illegal XhoI site found at 804
    Illegal XhoI site found at 1005
    Illegal XhoI site found at 1248
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1164
    Illegal NgoMIV site found at 1471
    Illegal NgoMIV site found at 1698
    Illegal NgoMIV site found at 1841
    Illegal NgoMIV site found at 2231
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 648
    Illegal BsaI site found at 1035
    Illegal BsaI site found at 1041
    Illegal BsaI site found at 1611
    Illegal BsaI site found at 1975
    Illegal BsaI.rc site found at 2342
    Illegal SapI site found at 995


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