T7
Part:BBa_K921002
Designed by: Eric Pederson Group: iGEM12_Carnegie_Mellon (2012-10-01)
Revision as of 23:43, 1 October 2012 by Enpederson (Talk | contribs)
T7 RNAP + IPTG->PoPs (Mutant III)
This is a mutant T7 promoter that includes a lacO sequence. This is repressible by the LacI protein and is induced in the presence of IPTG. The promoter sits in the 5' region of the gene of interest and initiates transcription when the cell expresses T7 RNA polymerase. Be sure you are using a cell strain that includes this gene (DE3).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Functional Parameters
| direction | Forward |
| efficiency | 160% |
| negative_regulators | LacI (Hypothesized LacIQ compatible) |
[edit]
Categories
Parameters
//chassis/bacteriophage/t7
//function/regulation/transcriptional
//plasmid/expression/t7
//promoter
//regulation/negative
//function/regulation/transcriptional
//plasmid/expression/t7
//promoter
//regulation/negative
| direction | Forward |
| efficiency | 160% |
| negative_regulators | LacI (Hypothesized LacIQ compatible) |