Part:BBa_K395704:Experience

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Applications of BBa_K395704

Data sheet for zeaxanthin synthesis BioBricks (This part applies to ①)

These parts contain crtZ (β-carotene hydroxylase) under inducible promoter control. This enzyme is responsible for the conversion of β-carotene into zeaxanthin. Our team designed some BioBricks to do this conversion. Characterization of these BioBricks has been performed in E. coli strain MG1655. (→[http://2010.igem.org/Team:Tokyo_Tech/Project/Apple_Reporter more information])

User Reviews

UNIQd6ccca263d7ee228-partinfo-00000000-QINU UNIQd6ccca263d7ee228-partinfo-00000001-QINU

Team WashU iGEM 2012 Characterization of Part BBa_K39504:

The construct that Tokyo Tech built is composed of two parts:
1. CrtZ which is needed to cleave beta-carotene into zeaxanthin. This component is repressed in the presence of glucose and
induced in the presence of arabanose through a pbad/arac promoter.
2. All the enzymes needed to produce beta-carotene from FPP in E. coli. Expression of these enzymes is regulated by a lacI repressed promoter, which is induced in the presence of lactose.
can be viewed below.





It can be observed from the graph that the groups with 2% arabanose are inhibited in their growth throughout the time measurements were taken, both with and without IPTG. It can also be seen that the un-induced group had the highest OD600 at 3.75hrs suggesting that overproduction of the enzymes coded for by BBa_395704 does have a deleterious effect on the cells.