Regulatory

Part:BBa_K799005

Designed by: Jiarui Wang   Group: iGEM12_Johns_Hopkins-Wetware   (2012-09-23)
Revision as of 21:15, 1 October 2012 by Danielw9 (Talk | contribs) (→‎=Characterization)

Ethanol/Stress Inducible YML070W Promoter

____ promoter shows an increase in protein expression when exposed to __% ethanol.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Characterization

ProA3 fluoro.png

This is fluorescence data for the expression of GFP under the control of YJL200C, our ethanol induced promoter. Fluorescence can be seen soon after ethanol induction. Furthermore, the fluorescence plateaus but remains significant till our last time point of 230 minutes.

Plasmid Map

[[]]


Sequencing

[[]]

This is the sequencing result for colony 1. Colony 1 is a perfect match to the genomic DNA promoter.

[edit]
Categories
Parameters
None