Generator
Part:BBa_K917001:Design
Designed by: Chris French Group: iGEM12_Edinburgh (2012-06-29)
ars promoter plus cscA sucrose assimilation gene
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1042
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 255
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Made by combining J33203 with K917000
Source
This part consists of the E. coli chromosomal ars promoter and arsR repressor gene (J33201), which together comprise a promoter system induced by sodium arsenate, plus the lacZ' minigene (J33202) and the cscA sucrose hydrolase gene from an E. coli O157 strain (K917000).
References
Jahreis, K., Bentler, L., Bockmann, J., Hans, S., Meyer, A., Siepelmeyer, J., et al. (2002). Adaptation of sucrose metabolism in the Escherichia coli Wild-Type Strain EC31132. Journal of Bacteriology, 5307-5316.