Regulatory

Part:BBa_K799009

Designed by: Jiarui Wang   Group: iGEM12_Johns_Hopkins-Wetware   (2012-09-23)
Revision as of 21:02, 1 October 2012 by Danielw9 (Talk | contribs) (→‎Sequencing)

Ethanol/Stress Inducible YMR251W-A Promoter

____ promoter shows an increase in protein expression when exposed to __% ethanol.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 120
    Illegal PstI site found at 277
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 169
    Illegal NheI site found at 355
    Illegal PstI site found at 277
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 120
    Illegal PstI site found at 277
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 120
    Illegal PstI site found at 277
  • 1000
    COMPATIBLE WITH RFC[1000]


Plasmid Map

[[]]


Sequencing

ProA5 sequencing.png

This is the sequencing result for YMR251W-A. As can be seen, it is a perfect match to the genomic DNA promoter.

[edit]
Categories
Parameters
None