Translational_Unit

Part:BBa_K917000:Design

Designed by: Chris French   Group: iGEM12_Edinburgh   (2012-06-19)
Revision as of 20:37, 1 October 2012 by Pandeli (Talk | contribs) (→‎Design Notes)

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cscA sucrose hydrolase from E. coli 157:H7 Sakai


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 265
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Due to the pathogenic nature of the source strain, purified genomic DNA was obtained from a veterinary laboratory to use as a PCR template, and the E. coli O157:H7 strain was never present in viable form in our laboratory. Note that the native RBS is included.

Source

Escherichia coli O157:H7 strain Sakai genomic DNA

References

Jahreis, K., Bentler, L., Bockmann, J., Hans, S., Meyer, A., Siepelmeyer, J., et al. (2002). Adaptation of sucrose metabolism in the Escherichia coli Wild-Type Strain EC31132. Journal of Bacteriology, 5307-5316