Translational_Unit

Part:BBa_K917004:Design

Designed by: Elitsa Peeva   Group: iGEM12_Edinburgh   (2012-09-20)
Revision as of 20:23, 1 October 2012 by Pandeli (Talk | contribs) (→‎Design Notes)

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nfsI nitroreductase gene from Enterobacter cloacae


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 581
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part includes the native ribosome binding site. The original sequence (http://www.ncbi.nlm.nih.gov/nuccore/M63808.1) had a PstI restriction site however the cloned gene did not have PstI restriction site ( confirmed trough sequencing and restriction digest).

Source

Enterobacter cloacae genomic DNA

References

Nicklin, C. E., & Bruce, N. C. (1998). Aerobic degradation of 2,4,6-Trinitrotoluene by Enterobacter cloaceae PB2 and by Pentaerythritol tetranitrate reductase. Applied and environmental microbiology , 2864-2868.

Nillius, D., Muller, J., & Muller, N. (2011). Nitroreductase (GlNR1) increases susceptibility of Giardia lamblia and Escherichia coli to nitro drugs. Journal of antimicrobial chemotherapy, 1029-1035.