Plasmid_Backbone

Part:BBa_K799025

Designed by: Daniel Wolozny   Group: iGEM12_Johns_Hopkins-Wetware   (2012-09-25)
Revision as of 19:10, 1 October 2012 by Danielw9 (Talk | contribs) (→‎Plasmid Map)

pSB1C3 yGG ORF acceptor vector

The original psb1c3 backbone was modified in order to allow for Golden Gate enabled parts to easily become biobricks.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XhoI site found at 1012
    Illegal XhoI site found at 1904
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


Plasmid Map

CDS Acceptance Vector Plasmid Map.png

Sequencing

[[]]

This is the sequencing result for colony 1. Colony 1 is a perfect match to the genomic DNA promoter.

[edit]
Categories
Parameters
None