Plasmid_Backbone

Part:BBa_K799025

Designed by: Daniel Wolozny   Group: iGEM12_Johns_Hopkins-Wetware   (2012-09-25)
Revision as of 19:09, 1 October 2012 by Danielw9 (Talk | contribs)

pSB1C3 yGG ORF acceptor vector

The original psb1c3 backbone was modified in order to allow for Golden Gate enabled parts to easily become biobricks.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XhoI site found at 1012
    Illegal XhoI site found at 1904
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


Plasmid Map

[[]]


Sequencing

[[]]

This is the sequencing result for colony 1. Colony 1 is a perfect match to the genomic DNA promoter.

[edit]
Categories
Parameters
None