Coding
ATF1

Part:BBa_J45006

Designed by: Veena Venkatachalam   Group: iGEM06_MIT   (2006-06-07)
Revision as of 22:14, 7 June 2006 by Veenav (Talk | contribs) (→‎Usage and Biology)

alcohol acetyltransferase I (ATF1)

From S. cerevisiae (yeast)

Usage and Biology

  • The gene ATF1 encodes the enzyme alcohol acetyltransferase I (AATase I), which produces acetate esters from acetyl CoA and alcohols. We will be interested in using AATase I to catalyze the reaction of isoamyl alcohol -> isoamyl acetate; isoamyl acetate is a volatile ester with a strong banana-like scent. (The related enzyme AATase II, which is encoded by the gene ATF2, performs a similar function...but it is less efficient.)
  • Horton et al. reported a yield of 1.8mM isoamyl acetate 40 hours after the addition of 10mM isoamyl alcohol to cultured E. coli expressing the ATF1 gene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 99
    Illegal BamHI site found at 1426
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 526
    Illegal SapI.rc site found at 1534


[edit]
Categories
//function/odor
Parameters
None