Coding
dszB

Part:BBa_K902052

Designed by: Lisa Oberding, Ali Honarmand   Group: iGEM12_Calgary   (2012-10-01)
Revision as of 18:35, 1 October 2012 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


HBPS desulfinase (DszB) from R. erythropolis (mutated for improved activity)

DszB - HBPS desulfinase which carries out the last step of the 4S pathway, converting 2-hydroxybiphenyl-2-sulfinic acid (HBPS) to 2-hydroxybiphenyl. The sequence has been mutated Y63F to increase enzyme activity.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 426
    Illegal XhoI site found at 418
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 133
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None