Generator

Part:BBa_K749015

Designed by: Takuya Akutsu   Group: iGEM12_TMU-Tokyo   (2012-09-26)
Revision as of 15:54, 1 October 2012 by T.acts (Talk | contribs)

formate dehydrogenase device

formate dehydrogenase device

Biosafety_Level1.png Safety.png

Constant protein generator of formaldehyde dehydrogenase 4 (FDH4) from Methylobacterium extroquens. FDH4 is composed of α-subunit and β-subunit. This parts consists both of them.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2297
    Illegal PstI site found at 2009
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2297
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal PstI site found at 2009
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2297
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2297
    Illegal PstI site found at 2009
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2297
    Illegal PstI site found at 2009
    Illegal NgoMIV site found at 1245
    Illegal NgoMIV site found at 1508
    Illegal NgoMIV site found at 1696
    Illegal NgoMIV site found at 1736
    Illegal NgoMIV site found at 1866
    Illegal NgoMIV site found at 2246
    Illegal NgoMIV site found at 2497
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 283
    Illegal BsaI site found at 1573
    Illegal BsaI.rc site found at 419
    Illegal BsaI.rc site found at 1112
    Illegal BsaI.rc site found at 1916
    Illegal BsaI.rc site found at 2434
    Illegal BsaI.rc site found at 2611
    Illegal SapI site found at 559
    Illegal SapI.rc site found at 121


[edit]
Categories
Parameters
None